On the Structure of Bovine Pancreatic Ribonuclease B
نویسندگان
چکیده
Ribonuclease B is a constituent of the zymogen granules of bovine pancreatic acinar cells and is secreted in the pancreatic juice of cattle (2). Its isolation from bovine pancreatic juice has been described (3). The enzyme is a glycoprotein that possesses an amino acid composition indistinguishable from that of ribonuclease A and contains carbohydrate to the extent of 6 residues of mannose and 2 residues of glucosamine per molecule. The experiments to be described in the present communication were designed to provide preliminary structural information about the protein and, in particular, to furnish an understanding of the distribution of the carbohydrate in the molecule. The experiments have indicated that the carbohydrate content is accounted for by a single covalently bonded oligosaccharide moiety attached at an aspartic acid or asparagine residue and have provided further presumptive evidence that the structure of the protein is otherwise identical with that of ribonuclease A.
منابع مشابه
The Isolation of Ribonuclease B, a Glycoprotein, from Bovine Pancreatic Juice*
Greene (1) found that the proteins of bovine pancreatic juice and pancreatic zymogen granules contain identical concentrations of a ribonuclease that on chromatography over Amberlite IRC-50 exhibits the behavior of the enzyme previously called (2, 3) ribonuclease B. The present report deals with the isolation of ribonuclease B from bovine pancreatic juice and provides a description of some of i...
متن کاملThe isolation of ribounclease B, a glycoprotein, from bovine pancreatic juice.
Greene (1) found that the proteins of bovine pancreatic juice and pancreatic zymogen granules contain identical concentrations of a ribonuclease that on chromatography over Amberlite IRC-50 exhibits the behavior of the enzyme previously called (2, 3) ribonuclease B. The present report deals with the isolation of ribonuclease B from bovine pancreatic juice and provides a description of some of i...
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Ribonuclease B is a constituent of the zymogen granules of bovine pancreatic acinar cells and is secreted in the pancreatic juice of cattle (2). Its isolation from bovine pancreatic juice has been described (3). The enzyme is a glycoprotein that possesses an amino acid composition indistinguishable from that of ribonuclease A and contains carbohydrate to the extent of 6 residues of mannose and ...
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